Polarised expression of human intestinal iV-benzoyl-L-tyroxyl-p-aminobenzoic acid hydrolase (human meprin) a and ß subunits in Madin-Darby canine kidney cells
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Human meprin beta: O-linked glycans in the intervening region of the type I membrane protein protect the C-terminal region from proteolytic cleavage and diminish its secretion.
Human meprin (hmeprin; N -benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase; EC 3.4.24.18) is a member of the astacin family of zinc metalloendopeptidases. The major site of expression is the brush border membrane of small intestinal and kidney epithelial cells. The enzyme is a type I integral membrane protein composed of two distinct subunits, alpha and beta, which are linked by disulphide bridg...
متن کاملMeprin A metalloproteinase and its role in acute kidney injury.
Meprin A, composed of α- and β-subunits, is a membrane-associated neutral metalloendoprotease that belongs to the astacin family of zinc endopeptidases. It was first discovered as an azocasein and benzoyl-l-tyrosyl-p-aminobenzoic acid hydrolase in the brush-border membranes of proximal tubules and intestines. Meprin isoforms are now found to be widely distributed in various organs (kidney, inte...
متن کاملExpression Profile of Drug and Nutrient Absorption Related Genes in Madin-Darby Canine Kidney (MDCK) Cells Grown under Differentiation Conditions
The expression levels of genes involved in drug and nutrient absorption were evaluated in the Madin-Darby Canine Kidney (MDCK) in vitro drug absorption model. MDCK cells were grown on plastic surfaces (for 3 days) or on Transwell® membranes (for 3, 5, 7, and 9 days). The expression profile of genes including ABC transporters, SLC transporters, and cytochrome P450 (CYP) enzymes was determined us...
متن کاملThe cytoplasmic domain of the interleukin-6 receptor gp80 mediates its basolateral sorting in polarized madin-darby canine kidney cells.
The IL-6 receptor complex is expressed in different polarized epithelial cells such as liver hepatocytes and intestinal cells. It consists of two subunits: gp80, which binds the ligand, and gp130, which is responsible for signal transduction. In stably transfected Madin-Darby canine kidney (MDCK) cells we have studied the localization of the human IL-6 receptor subunits and found that gp80 and ...
متن کاملInterleukin-6 Cleavage by Meprin Metalloproteinases A and B 1 Meprin Metalloproteases Inactivate Interleukin-6*
Meprins have been implicated in the pathogenesis of several inflammatory diseases including inflammatory bowel disease, in which the cytokine interleukin-6 (IL-6) is a prominent effector molecule. Because IL-6 levels are markedly elevated in meprin α and α/β knockout mice in an experimental model of IBD, the interaction between meprins and IL-6 were studied. The results demonstrate that rodent ...
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تاریخ انتشار 2017